SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-14 01:28:46

description

threonine synthase

threonine synthase, has a minor threonine dehydratase ([[protein|IlvA]]) activity

locus

BSU32250

BSU_32250

geneLength

1056

1059

outlinks

bsu

BSU32250

BSU_32250

Gene

Coordinates

3,313,770 → 3,314,828

3,313,770 3,314,828

The protein

Catalyzed reaction/ biological activity

O-phospho-L-homoserine + H2O = L-threonine + phosphate (according to Swiss-Prot)

O-phospho-L-homoserine + H2O --> L-threonine + phosphate (according to UniProt)

has additional threonine dehydratase activity [pubmed|27260660]

The protein

Protein family

serine/threonine dehydratase family (according to Swiss-Prot)

threonine synthase family (single member, according to UniProt)

The protein

Structure

[PDB|1UIN] (from ''Thermus thermophilus'', 51% identity, 69% similarity) [Pubmed|12952961]

[PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]

[PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]

Biological materials

Mutant

1A773 ( ''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab

GP3030 Δ[[gene|thrC]]::''spc'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab

BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

References

12107147, 3098560, 24163341, 15378759, 27260660

12107147, 3098560, 24163341, 15378759, 27260660, 30830751, 4630852, 4199513, 32743959

Gene

Phenotypes of a mutant

auxotrophic for threonine [pubmed|32743959]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

PLP