2017-8-11 16:0:32025-05-14 01:28:46
description
threonine synthase
threonine synthase, has a minor threonine dehydratase ([[protein|IlvA]]) activity
locus
BSU32250
BSU_32250
geneLength
1056
1059
outlinks
bsu
BSU32250
BSU_32250
Gene
Coordinates
3,313,770 → 3,314,828
3,313,770 3,314,828
The protein
Catalyzed reaction/ biological activity
O-phospho-L-homoserine + H2O = L-threonine + phosphate (according to Swiss-Prot)
O-phospho-L-homoserine + H2O --> L-threonine + phosphate (according to UniProt)
has additional threonine dehydratase activity [pubmed|27260660]
The protein
Protein family
serine/threonine dehydratase family (according to Swiss-Prot)
threonine synthase family (single member, according to UniProt)
The protein
Structure
[PDB|1UIN] (from ''Thermus thermophilus'', 51% identity, 69% similarity) [Pubmed|12952961]
[PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]
[PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]
Biological materials
Mutant
1A773 ( ''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab
GP3030 Δ[[gene|thrC]]::''spc'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab
BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
References
Gene
Phenotypes of a mutant
auxotrophic for threonine [pubmed|32743959]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Cofactors]
PLP